منابع مشابه
Enzymatic mutation detection in the P53 gene.
BACKGROUND The enzymatic mutation detection (EMD) assay uses the bacteriophage resolvase T4 endonuclease VII, which cleaves preformed heteroduplex molecules at mismatch sites, forming two shorter fragments that can be resolved by gel electrophoresis. The method can be used to detect single and multiple base changes, as well as insertions and deletions. METHODS The sensitivity, specificity, an...
متن کاملAutomated fluorescent analysis procedure for enzymatic mutation detection.
The Enzymatic Mutation Detection (EMD) assay detects mutations or polymorphisms in DNA. The assay procedure takes <1 h and is followed by electrophoretic detection. We report an automated procedure, using fluorescently labeled probe and quantitative analysis on the ABI Prism 377 DNA Sequencer, that improves on earlier methods (1, 2) by eliminating the need for sample purification, shortening th...
متن کاملPharmacogenetic Application of High-Throughput Mutation Detection and Genotyping Technologies
Pharmacogenetics is a scientific discipline that examines the genetic basis for individual variations in response to therapeutics. Pharmacogenetics promises to develop individualized medicines tailored to patients’ genotypes. However, identifying and genotyping a vast number of genetic polymorphisms in large populations also pose a great challenge. This article will address the recent technolog...
متن کاملEnzymatic mutation detection method evaluated for detection of p53 mutations in cDNA from breast cancers.
BACKGROUND Rapid, reproducible, and easily run methods with high sensitivity and specificity are required for mutation screening of clinical samples. We evaluated the Enzymatic Mutation Detection (EMD(TM)) method by analysis of archival cDNA from 203 breast cancer patients and comparison with results of cDNA-based sequencing of the tumor suppressor gene p53. METHODS The EMD technology uses th...
متن کاملSelective Enzymatic Amplification of a2-Globin DNA for Detection of the Hemoglobin Constant Spring Mutation
Blood, Vol 73, No 7 (May 15), 1989: pp 1987-1992 1987 Hemoglobin Constant Spring is an elongation mutation of the a2-globin locus that results in a thalassemic phenotype. It has a high prevalence in Asian populations. When inherited with other a-thalassemia determinants, the Constant Spring gene has the potential to cause severe forms of a-thalassemia. Accurate diagnosis of the condition with s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2005
ISSN: 0736-6205,1940-9818
DOI: 10.2144/05385rv01